Research Paper Volume 12, Issue 13 pp 13618—13632

Figure 5. Knockdown of SERCA2a attenuates YAP-induced protective effects on mitochondria and ERS in I/R-treated cardiomyocytes. SERCA2a siRNA was transfected into cardiomyocytes infected with ad-YAP in the presence of mI/R injury. (A–C) The activity of glutathione (GSH), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were measured via enzyme-linked immunosorbent assay (ELISA). (D) ATP production was determined through ELISA. (E) The levels of glucose in the medium were determined through ELISA. (F, G) Quantitative polymerase chain reaction (qPCR) assay was used to analyze the mRNA levels of PERK and CHOP. (H, I) ELISA was used to detect the activation of caspase-12 and caspase-3. *P<0.05.