Figure 4.Activation of the YAP/SERCA2a pathway attenuates ERS in I/R-treated cardiomyocytes. (A, B) Cardiomyocytes were transfected with ad-SERCA2a and ad-YAP to overexpress SERCA2a and YAP, respectively. Quantitative polymerase chain reaction (qPCR) assay was used to analyze the mRNA levels of PERK and CHOP in cardiomyocytes transfected with ad-SERCA2a or ad-YAP in the presence of mI/R injury. (C) Quantification of relative calcium immunofluorescence intensity in cardiomyocytes transfected with ad-SERCA2a or ad-YAP in the presence of mI/R injury (D) Immunofluorescence assay was used to assess the baseline calcium overload in cardiomyocytes. (E, F) Enzyme-linked immunosorbent assay (ELISA) was used to detect the activation of caspase-12 and caspase-3. Scale bar, 85 μm.*P<0.05.
