Research Paper Volume 12, Issue 13 pp 13618—13632

Figure 3. Activation of the YAP/SERCA2a pathway reduces mitochondrial damage in I/R-treated cardiomyocytes. (A, B) Immunofluorescence assay was used to detect the levels of mitochondrial ROS in cardiomyocytes transfected with ad-SERCA2a or ad-YAP in the presence of mI/R injury. (C–E) The activities of glutathione (GSH), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were measured via enzyme-linked immunosorbent assay (ELISA) in cardiomyocytes transfected with ad-SERCA2a or ad-YAP in the presence of mI/R injury. (F, G) Mitochondrial membrane potential was measured using JC-1 staining. (H) ATP production was analyzed through ELISA. (I) The levels of glucose in the medium were determined by ELISA. *P<0.05.