Research Paper Volume 12, Issue 13 pp 13618—13632
The YAP/SERCA2a signaling pathway protects cardiomyocytes against reperfusion-induced apoptosis
Figure 2. SERCA2a is transcriptionally regulated by YAP in cardiomyocytes. (A, B) Cardiomyocytes were transfected with ad-SERCA2a and ad-YAP to overexpress SERCA2a and YAP, respectively. Quantitative polymerase chain reaction (qPCR) assay was used to analyze the mRNA levels of YAP and SERCA2a in cardiomyocytes subjected to mI/R injury. (C, D) The qPCR assay was used to detect the mRNA levels of YAP and SERCA2a in cardiomyocytes transfected with SERCA2a siRNA or YAP siRNA. (E, F) Immunofluorescence assay was used to detect the expression of SERCA2a and YAP in cardiomyocytes subjected to mI/R using anti-SERCA2a (pink) and anti-YAP (green) antibodies, respectively. Scale bars, 95 μm. Left panels show quantification of the expression of SERCA2a and YAP.*P<0.05.