Research Paper Volume 12, Issue 13 pp 13618—13632

Figure 1. Overexpression of YAP or SERCA2a attenuates I/R-induced cardiomyocyte apoptosis. (A, B) Quantitative polymerase chain reaction (qPCR) assay was used to analyze the mRNA levels of YAP and SERCA2a in cardiomyocytes subjected to mI/R injury. SERCA2a adenovirus (ad-SERCA2a) and YAP adenovirus (ad-YAP) were transfected into cardiomyocytes to overexpress SERCA2a and YAP, respectively. (C) The MTT assay was used to detect the cardiomyocyte viability and (D, E) PI staining was used to assess the number (percentage) of apoptotic cells. (F, G) The qPCR assay was used to analyze the mRNA levels of YAP and SERCA2a in cardiomyocytes transfected with ad-SERCA2a or ad-YAP under physiological conditions. *P<0.05.