Research Paper Volume 12, Issue 17 pp 16820—16836
Neutrophil extracellular traps amplify neutrophil recruitment and inflammation in neutrophilic asthma by stimulating the airway epithelial cells to activate the TLR4/ NF-κB pathway and secrete chemokines
Figure 5. Degradation of NETs by DNase I has no effect on chemokine expression and neutrophil migration in vitro. (A, B) After treatment of HBEs and A549 cells with DNase I and NETs simultaneously, CXCL1, CXCL2, and CXCL8 levels in the supernatant were analyzed by Luminex and RT-qPCR. (C and D) After treatment of HBEs and A549 cells with DNase I and NETs simultaneously, the supernatant was collected and added into the lower chamber. Neutrophils were cultured in the upper chamber and neutrophil migration was analyzed by transwell after cell culture supernatant was induced for 48 h (200X). #: P<0.05, ##: P<0.01, ###: P<0.001, ####: P<0.0001 vs control group. *: P<0.05, **: P<0.01, ***: P<0.001: ****, P<0.0001 vs NA group. ns = no statistical difference vs NA or control group.