Research Paper Volume 12, Issue 17 pp 16820—16836
Neutrophil extracellular traps amplify neutrophil recruitment and inflammation in neutrophilic asthma by stimulating the airway epithelial cells to activate the TLR4/ NF-κB pathway and secrete chemokines
Figure 3. Blocking the TLR4/NF-κB pathway with TAK-242 reduced the expression of chemotactic factors and alleviate airway inflammation in NA mice. (A) Flow chart for intraperitoneal administration of TAK-242. (B) HE staining results showed that TAK-242 treatment could alleviate inflammation (200X). (C) p65 NF-κB and p-p65 NF-κB expression was measured by western blotting. (D) p65 NF-κB expression was measured by immunohistochemistry (200X). (E–G) Total cells, differential cells, and total protein were measured in the BALF of NA mice. (H) Airway resistance was measured after methacholine treatment. (I) CXCL1, CXCL2, and CXCL8 expression in the BALF of NA mice was measured by ELISA. (J) CXCL1, CXCL2, and CXCL8 expression was measured by Immunohistochemistry in the lung tissues of NA mice. #: P<0.05, ##: P<0.01, ###: P<0.001, ####: P<0.0001 vs control group. *: P<0.05, **: P<0.01: ***: P<0.001, ****: P<0.0001 vs NA group. ns = no statistical difference vs NA group.