Research Paper Volume 12, Issue 17 pp 16820—16836
Neutrophil extracellular traps amplify neutrophil recruitment and inflammation in neutrophilic asthma by stimulating the airway epithelial cells to activate the TLR4/ NF-κB pathway and secrete chemokines
Figure 2. Inhibition of NET formation alleviated neutrophilic airway inflammation in NA model, rather than degrading the NETs DNA. (A) Flow chart for sivelestat or DNase I administration intraperitoneally. (B) HE staining results showed that sivelestat could alleviate inflammation while DNase I could not (200X). (C–E) Total cells, differential count of cells, and total protein were measured after treatment with sivelestat or DNase I in the BALF of NA mice. (F) Airway resistance was measured after methacholine treatment. (G) Concentration of CXCL1, CXCL2 and CXCL8 in the BALF supernatant of NA mice was measured by ELISA. (H) CXCL1, CXCL2, and CXCL8 expression was measured by Immunohistochemistry (200X). #: P<0.05, ##: P<0.01, ###: P<0.001, ####: P<0.0001 vs control group. *: P<0.05, **: P<0.01, ***: P<0.001, ****: P<0.0001 vs NA group. ns = no statistical difference vs NA group.