Research Paper Volume 12, Issue 13 pp 13422—13436

Figure 1. CPPs alleviated cognitive impairments in APP/PS1 mice. 5.5-month-old C57/BL6 mice served as WT control and were treated with IG administration of normal saline for one month. 5.5-month-old APP/PS1 mice were treated with one-month IG administration of 100 or 300 mg/kg of CPPs, or with normal saline as positive control. At the age of 6.5 month, all the mice were assessed in behavioral tests. (A–D) Open field tests and Elevated plus maze tests were used to assess the anxiety-like behavior of mice. (A) Representative trajectory, (B) Center duration, (C) Open-Arm entry, (D) Open-Arm duration. (E, F) NOR experiment was performed to assess the recognition index. (E) Recognition index between object A and B were detected. Recognition index were recorded in 5 min. After 24 h, object C was used instead of object B. (F) Recognition index between object A and C were detected. (G–L) Morris water maze (MWM) test was performed to evaluate the spatial memory of mice. (G) Escape latency of during the 5 days trial. (H) Representative trajectory of mice in the probe trial. (I) The first passage time of the platform in the target quadrant in 60 sec after removing the platform. (J) The number of crossing times. (K) The time spent in target quadrant. (L) The total swimming distance of each group. P value significance is calculated from a one-way ANOVA or two-way ANOVA. *P < 0.05, **P < 0.01, vs. WT control mice, #P < 0.05, ##P < 0.01 vs. positive control (APP/PS1 mice without CPPs treatment), n = 6.