Research Paper Volume 12, Issue 13 pp 13255—13280

Figure 5. miR-1271-5p functions as a tumor suppressor in vitro. (A) qRT-PCR analysis of miR-1271-5p expression in RWPE-1, PC-3, and DU145 cells. (B, C) CCK-8 assay determined the cell viability in PC-3 and DU145 cells treated with miR-1271-5p mimic. (D, E) Representative images and quantification of colony formation assays in PC-3 and DU145 cells treated with miR-1271-5p mimic. (F, G) Representative images and quantification of EdU assays in PC-3 and DU145 cells treated with miR-1271-5p mimic. Scale bars: 100 μm. (H, I) Representative images and quantification of wound healing assays in PC-3 and DU145 cells treated with miR-1271-5p mimic. Scale bars: 200 μm. (J, K) Representative images and quantifications of transwell assays in PC-3 and DU145 cells treated with miR-1271-5p mimic. Scale bars: 100 μm. Data are displayed as mean ± SD. *p < 0.05; **p < 0.01.