Research Paper Volume 12, Issue 13 pp 13038—13058

Figure 2. circMET exerts oncogenic effects in NSCLC cell lines. (A) Relative expression of circMET in several human NSCLC cell lines was examined by qRT-PCR normalized to GAPDH expression. (B) and (C) The efficiency of transfection in the NCI-H1299 and NCI-H460 cell lines was confirmed by qRT-PCR analysis, whereas the circMET expression was unchanged. GAPDH was used as a control for loading. (D) and (E) Cell proliferation in NSCLC cells with different circMET expression was assessed by CCK-8 (D) and colony formation assays (E). (F) and (G) Cell invasion and migration abilities were assessed by Matrigel transwell (F) and wound healing assays (G), respectively. Scale bar: 100 μm. The data was represented as the mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001. NS: no significant.