Priority Research Paper Volume 12, Issue 11 pp 10041—10058

Figure 6. Neuronal knockdown of Ldh delays age-related neurodegeneration. (A) Ldh mRNA levels were significantly reduced in the heads of 55-day old elav^ts>Ldh^RNAi compared to age-matched elav^ts>RNAi^control flies (n=3; Unpaired t-test with Welch’s correction *p<0.05). (B) Survival curves of elav^ts>Ldh^RNAi (n=187) and elav^ts>RNAi^control (n=195). Median lifespan was significantly extended in elav^ts>Ldh^RNAi flies compared to control (Gehan-Breslow-Wilcoxon test; p<0.0001; see also Supplementary Table 3). (C, D) Representative brain section images of 75-day old (C) elav^ts>RNAi^control and (D) elav^ts>Ldh^RNAi flies (arrowheads indicate vacuoles). (E, F) Graphs showing that the average area (E) and number (F) of vacuoles were significantly lower in the brains of 75-day old elav^ts>Ldh^RNAi flies (n=48) compared to controls (n=46). Statistical significance determined by unpaired t-test with Welch’s correction *p<0.05. Bars indicated SEM.