Research Paper Volume 12, Issue 20 pp 20047—20068
CUEDC1 inhibits epithelial-mesenchymal transition via the TβRI/Smad signaling pathway and suppresses tumor progression in non-small cell lung cancer
Figure 7. CUEDC1 decreases cell proliferation and promotes cell apoptosis in NSCLC cells. (A) Colony-forming efficiency was determined in H1299/shRNA-CUEDC1 cells, A549/shRNA-CUEDC1 cells, H460/CUEDC1 cells, and corresponding control cells (vector control). (B) Cell proliferation was determined by the EdU-incorporation assay in H1299/shRNA-CUEDC1 cells, A549/shRNA-CUEDC1 cells, H460/CUEDC1 cells, and corresponding control cells (vector control). (C) Flow cytometric analysis of apoptosis in H1299/shRNA-CUEDC1 cells, A549/shRNA-CUEDC1 cells, H460/CUEDC1 cells and negative control cells by Annexin-V and 7-AAD staining. A representative flow profile is presented (upper), and summary of % for Annexin V-positive cells is shown (below). (D) Western blotting detected the expression of proteins that participate in the cell cycle and apoptosis. The data are expressed as the mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001.