Research Paper Volume 12, Issue 20 pp 20047—20068

Figure 3. CUEDC1 expression was negatively correlated with EMT process in NSCLC tumor tissues and cell lines. (A) IHC detected the expression of CUEDC1, E-cadherin, N-cadherin in lung adenocarcinoma and lung squamous cell carcinoma tissues (magnification, ×100 and ×400). (B) A significant correlation between CUEDC1 expression and E-cadherin expression (upper panel), N-cadherin expression (lower panel) in NSCLC tissues. P values were calculated using the χ2 test. (C) The expression of EMT markers in CUEDC1-shRNA cells and control cells of H1299 and A549 cell lines. The expression of EMT markers in H460-CUEDC1 cells and control cells. (D) The protein levels and localization of EMT markers were observed by immunofluorescence in H1299 cells and A549 cells. Representative fluorescein immunocytochemical staining is depicted with E-cadherin (green), N-cadherin (red), Vimentin (red), and nuclear DAPI (blue). Experiments were performed at least three times. The data are expressed as the mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001.