Figure 5. The activation of microglia and astrocytes in the hypothalamus. (A) Iba1 expression levels in hypothalamic microglia. The statistics analysis of the expression of Iba1 positive cells. (B) The expression levels of GFAP in hypothalamic astrocytes. (C) The statistics analysis of the expression of Iba1 positive cells. (D) The statistics analysis of the expression of GFAP positive cells. The scale bar is shown in the figure. vs control group, ▲▲p < 0.01. vs HFD group, ** p < 0.01. vs DC group, ## p < 0.01.