Research Paper Volume 12, Issue 11 pp 11071—11084
LINC01419 facilitates hepatocellular carcinoma growth and metastasis through targeting EZH2-regulated RECK
Figure 4. LINC01419 stabilizes EZH2 mRNA by recruiting FUS. (A, B) Showing RT-PCR and western blot assays used to examine EZH2 expression levels in HCC cells when LINC01419 was inhibited or overexpressed, respectively. (C) Luciferase reporter assay showing that LINC01419 did not affect EZH2 transcription. (D) FUS interaction with LINC01419 and EZH2-mRNA as validated by the RIP assay. (E) The estimated impact of LINC01419 down-regulation on FUS interaction with EZH2-mRNA using RIP assay. (F) The estimated impact of LINC01419 overexpression on FUS–interaction with EZH2 -mRNA using RIP assay. (G–H) A qRT-PCR assay used to examine the EZH2 expression level. (I) The degradation rate of EZH2-mRNA after treatment with actinomycin D. (J) Right panel: Correlation between LINC01419 expression level and EZH2 expression level examined by RT-PCR; Left panel: Correlation between RECK expression level and EZH2 expression level examined by RT-PCR.