Research Paper Volume 12, Issue 11 pp 10969—10982

Figure 7. P22077 relieves LPS-induced inflammatory response in mice. C57BL/6 mice (10 mice/group) were intraperitoneally injected with DMSO or P22077 (15 mg/kg), following challenged with PBS or LPS (10 mg/kg). Mice were sacrificed after 2 h. (A) TNF-α, IL-1β and IL-6 mRNA level in lung tissue were analyzed by Q-PCR. (B) Nitric oxide (NO) concentrations in the serum were examined by Griess assay. (C) TNF-α, IL-1β and IL-6 concentrations in the serum were examined by ELISA. (D) Left lung was collected for HE staining, and representative images were displayed. Scale bars, 100 μm. (E) Left lung was collected for immunohistochemistry staining of TRAF6. Scale bars, 100 μm. (F) Lung tissue was grinded and centrifuged, and the supernatant was used to analyzed the indicated proteins by immunoblot. Similar results were obtained from three independent experiments and data were presented as mean ± SD of one representative experiment. *P<0.05, **P<0.01, ***P<0.001 vs LPS-stimulated DMSO group (A–C).