Research Paper Volume 12, Issue 12 pp 11550—11567

Figure 6. ZFP36 is the direct target of miR-9-5p. (A) The putative miRNA binding sites in the ZFP36 sequence. (B) Direct target sites verified by the dual-luciferase reporter gene assay. (C) ZFP36 expression in HCC tissues and adjacent normal tissues detected via qRT-PCR. (D) Bivariate correlation analysis of the relationship between SENP3-EIF4A1 and ZFP36 expression level. (E) There was a significantly negative correlation between the expression level of SENP3-EIF4A1 and the expression level of miR-9-5p in the same paired HCC samples. (F) There was a significantly negative correlation between the expression level of ZFP36 and the expression level of miR-9-5p in the same paired HCC samples. (G) ZFP36 in HuH7 and Hep3b cells with SENP3-EIF4A1-Exos and/or miR-9-5p mimics examined via Western blotting. (H) ZFP36 in SMMC-7721 cells with SENP3-EIF4A1 siRNA-Exos and/or miR-9-5p inhibitor examined via Western blotting. Data are shown as mean ± SD. *P<0.05. All of the experiments were performed in triplicate.