Research Paper Volume 12, Issue 11 pp 10809—10826

Figure 3. LncRNA and mRNA expression profile changes between serious dural penetration and no-dural penetration in chordoma. (A) Volcano plot analysis of 2760 lncRNAs and 3988 mRNAs that are differentially expressed between no dural penetration group and serious dural penetration group; and red boxes represent ≥ 2-fold change difference and P ≤ 0.05. (B) Hierarchical clustering of all samples revealed the nonrandom partitioning of samples into two major groups: one group containing six no dural penetration samples and another group containing six serious dural penetration samples. Each column represents one sample and each row represents one lncRNA or mRNA probe set. (C) Transcriptional patterns of lncRNAs. Nature antisense: the lncRNA is transcribed from the antisense strand and overlapping with a coding transcript; Intronic antisense: the lncRNA is transcribed from the antisense strand without sharing overlapping exons; Intron sense-overlapping: the lncRNA is overlapping the intron of a coding transcript on the same genomic strand; Exon sense-overlapping: the lncRNA is overlapping the exon of a coding transcript on the same genomic strand; Intergenic: there are no overlapping or bidirectional coding genes nearby the lncRNA; Bidirectional: the lncRNA is oriented head to head to a coding gene within 1000 bp. Arrow represent transcription direction. (D) Subgroup analysis of altered lncRNAs in relation to their nearby coding genes.