Research Paper Volume 12, Issue 11 pp 10527—10543
Human amnion-derived mesenchymal stem cells promote osteogenic differentiation of human bone marrow mesenchymal stem cells via H19/miR-675/APC axis
Figure 6. APC inhibits Wnt/β-catenin pathway and HAMSCs-droved osteogenesis. (A, B) Protein levels of β-catenin, Cyclin D1 and c-Myc were assessed by western blot assay in NC and APC groups. (C) Relative mRNA expression of β-catenin was measured by RT-PCR analysis in NC and APC groups. (D) Immunofluorescence staining showed the β-catenin location in NC and APC groups. Scale bar, 20 μm. (E, F) Protein levels of ALP, RUNX2, OCN and OSX were assessed by western blot assay in NC and APC groups. (G) Relative mRNA expressions of ALP, RUNX2, OCN, and OSX were measured by RT-PCR analysis in NC and APC groups. Data are shown as mean ± SD. **P < 0.01.