Research Paper Volume 12, Issue 11 pp 10527—10543
Human amnion-derived mesenchymal stem cells promote osteogenic differentiation of human bone marrow mesenchymal stem cells via H19/miR-675/APC axis
Figure 2. LncRNA-H19 in HAMSCs promotes osteogenic differentiation of HBMSCs. (A) Protein levels of ALP, RUNX2, OCN, and OSX were assessed by western blot assay in HBMSCs, NC, H19, shNC and shH19 groups. (B) Relative mRNA expressions of ALP, RUNX2, OCN and OSX were measured by RT-PCR analysis in HBMSCs, NC, H19, shNC and shH19 groups. (C) ALP staining and activity in HBMSCs, NC, H19, shNC, and shH19 groups. Scale bar, 100 μm. (D) Alizarin red staining and quantification in HBMSCs, NC, H19, shNC, and shH19 groups. Scale bar, 1cm. Data are shown as mean ± SD. *P < 0.05 and **P < 0.01.