Research Paper Volume 12, Issue 11 pp 10398—10414

Figure 6. Effects of gonadectomy and TP replacement on mitochondrial complex V subunit expression in the substantia nigra of adult male rats. (A–E) The mRNA levels of ATP6, ATP8, ATP5C1, ATP5I and ATP5L were calculated using the 2^-ΔΔCt method. GAPDH was used as an internal control. (F–J) Representative Western blots of ATP6, ATP8, ATP5C1, ATP5I and ATP5L protein levels. (K–O) ATP6, ATP8, ATP5C1, ATP5I and ATP5L protein levels were quantified by comparing the band density of each protein to that of β-actin (endogenous control). Data are expressed as the mean ± S.D. (n=6 rats/group). ^*P<0.05, ^**P<0.01.