Research Paper Volume 12, Issue 12 pp 11517—11529

Figure 6. The hsa_circ_0004018 suppressed the proliferation and activation of HSCs through upregulating the expression of TEP1. (A) After transfection with TEP1 containing plasmid and the vector control, the RNA levels of TEP1 were detected by real-time PCR. The data are from three independent experiments. (B, C) After overexpression with TEP1, the growth curves of the HSCs were examined by CCK8 assay (B), while the expression of α-SMA and COL1A1 in HSCs was detected by western blotting (C). (D, E) The RNA levels (D) and the protein levels (E) were respectively detected after overexpression with hsa_circ_0004018. The vector was transfected as control. (F, G) After transfection with the indicated mimic hsa-miR-660-3p or negative control (NC) plus hsa_circ_0004018 or the vector control, the RNA levels (F) and the protein levels (G) of TEP1 in HSCs were respectively detected by real-time PCR and western blotting.