Research Paper Volume 12, Issue 12 pp 11517—11529

Figure 4. The hsa-miR-660-3p can promote the proliferation and activation of HSCs in vitro, and counteract with the function of hsa_circ_0004018. (A) It was detected the relative expression of hsa-miR-660-3p in the liver samples from CCl₄-induced liver fibrosis mice sacrificed at 0, 60 and 90 days after CCl₄ injection. (B, C) After transfection with mimic hsa-miR-660-3p (B) and inhibitor (C), the relative expression levels of hsa-miR-660-3p in HSCs were detected by real-time PCR. (D, E) After transfection with mimic hsa-miR-660-3p (D) and inhibitor (E), the growth curves of the HSCs were examined by CCK8 assay. (F, G) After transfection with mimic hsa-miR-660-3p (F) and inhibitor (G), the expression of α-SMA and COL1A1 in HSCs was detected by western blotting. (H, I) After transfection with the indicated mimic hsa-miR-660-3p or negative control (NC) plus hsa_circ_0004018 or the vector control, the growth curves of the HSCs were examined by CCK8 assay (H), while the expression of α-SMA and COL1A1 was detected by western blotting.