Research Paper Volume 12, Issue 12 pp 11517—11529

Figure 2. The overexpression of hsa_circ_0004018 promoted G0/1 cell cycle arrest of the primary HSCs through upregulating the expression of α-SMA and COL1A1. (A) The relative expression levels of hsa_circ_0004018 were detected by real-time PCR in the overexpressed HSCs. The vector was performed as control. (B, C) The EDU incorporating levels were measured in the hsa_circ_0004018 overexpressed HSCs and the control cells (B). The relative IOD values of the EDU incorporating of the indicated cells were statistically analyzed and presented as columns (C). Each group was independently examined three times. (D–F) The representative cell cycle distributions of the hsa_circ_0004018 overexpressed HSCs (E) and the control cells (D) were analysis by FACS. The cell distribution ratios of the G0/1 phase, the S phase and the G2/M phase of the indicated cells were statistically analyzed (F). (G, H) The relative RNA levels of α-SMA (G) and COL1A1 (H) of the hsa_circ_0004018 overexpressed HSCs and the control cells were detected by real-time PCR. (I) The expression of α-SMA and COL1A1 was detected by western blotting. (J) The expression of α-SMA was detected by immunofluorescence. (K) The expression of COL1A1 was detected by immunofluorescence.