Research Paper Volume 12, Issue 10 pp 9915—9934

Figure 5. The validation of circZNF139. (A) The circRNA-formed ability of ZNF139 evaluated by UCSC (http://genome.ucsc.edu/index.html) database. (B) Several circRNAs formed by ZNF139 were evidently differentially expressed in various tumor cells according to DeepBase (https://omictools.com/deepbase-tool) database. (C) ZNF139 (left panel) and circZNF139 (right panel) were both evidently upregulated in five BC cell lines (UC3, 5637, T24, EJ and J82) compared to one normal cell line SV-HUC-1 detected by qRT-PCR assay. (D) The RNA level of predicted circRNA and its control gene GAPDH were evaluated with or without RNase R treatment by qRT-PCR assay. The predicted circRNA, circZNF139, was resistant to RNase R treatment. *, P<0.05, **, P<0.01, ***, P<0.001.circ, circular; ZNF139, zinc finger with KRAB and SCAN domains 1; UCSC, University of California, Santa Cruz; BC, bladder cancer; qRT-PCR, quantitative real-time polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.