Figure 3A-F.QGS inhibits EMT in esophageal cancer cells. (A–C) Immunofluorescence images showing E-ca, N-ca, and Snail1 localization in ECA109 cells. QSG dose-dependently increased E-ca expression and inhibited N-ca and Snail1 expression. (D–F) Immunofluorescence images of E-ca, N-ca, and Snail1 in TE13 showing the same trend after QSG stimulation. Scale bars indicate 10 μm.
(G, H) Representative Western immunoblots. Bar graphs show significant dose-dependent increases in E-ca and decreases in N-ca and Snail1 relative protein density (normalized to β-act) after QSG stimulation compared to the unstimulated control group. Results are from three independent experiments. **p<0.01, E-ca relative protein density compared to control; &p<0.05, &&p<0.01, N-ca relative protein density compared to control; #p<0.05, ##p<0.01, Snail relative protein density compared to control.
