Research Paper Volume 12, Issue 12 pp 11485—11499

Figure 3. The effect of high glucose on the expression levels of CTRP13, p-CaMKKβ, CaMKKβ, p-AMPK, AMPK, LN and CAV-1 in rLSECs transfected by recombinant LV-CTRP13. (A) qRT-PCR analysis of CTRP13 mRNA. (B) qRT-PCR analysis of CaMKKβ mRNA. (C) qRT-PCR analysis of AMPKα mRNA. (D) qRT-PCR analysis of LN mRNA. (E) qRT-PCR analysis of CAV-1 mRNA. (A–E) The results were normalised to β-actin mRNA levels. (F) The protein expression levels of each group were detected using western blotting, and β-actin was used as a loading control. (G) Western blotting results showing relative CTRP13 expression. (H) Western blotting results showing relative CaMKKβ expression. (I) Western blotting results showing relative phos-CaMKKβ expression of CaMKKβ activation. (J) Western blotting results showing relative AMPKα expression. (K) Western blotting results showing relative phos-AMPKα expression of AMPKα activation. (L) Western blotting results showing relative LN expression. (M) Western blotting results showing relative CAV-1 expression. (F–M) β-actin (42 kDa) represents the loading control. All results are expressed as mean±S.D. from three independent experiments, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. control. ^##P < 0.01, ^###P < 0.001 vs the high glucose + LV-CON group, respectively.