Research Paper Volume 12, Issue 12 pp 11485—11499

Figure 1. Characterisation and identification of rat liver sinusoidal endothelial cells (rLSECs) in vitro. (A) The morphology of rLSECs under white light. The cells grew by static adherence and the morphology of rLSECs were polygon or fusiform, resembling cobblestones under inverted microscopy. (B) Representative images of immunofluorescence staining of CD31 in rLSECs. Cells were stained with anti-CD31 antibodies. CD31 is uniformly expressed in rLSECs. Fluorescence images were acquired at an original magnification (green, CD31 expression). (a. ×100; b. ×200)