Research Paper Volume 12, Issue 10 pp 9240—9259

Figure 3. BRD4 is a novel target for the prevention of macrophage senescence. THP-1 macrophages were incubated with or without LPS. The cells were then treated with the inhibitors JQ-1 or I-BET762. (A) SA-β-gal staining was performed and quantified. (B) The mRNA levels of the relative expression of SASP genes are shown in the cluster heatmaps. The histogram on the right shows the exact mRNA levels of IL-6 and CXCL1. (C) The protein levels of the senescence markers p53, p21, and p16 were evaluated by western blotting. (D) The immunofluorescence of THP-1 cells stained for p16 (green), BRD4 (green), and DAPI (blue) was observed by confocal microscopy. (E) Representative ORO staining and statistical data were used to analyze the lipid accumulation of THP-1 macrophages. The data all represent measurement data presented as the mean ± SD. The different groups were statistically analyzed using one-way ANOVA. Significant differences among the different groups are indicated as *p <0.05 vs. LPS; **p <0.01 vs. LPS; ***p <0.001 vs. LPS; ****p <0.0001 vs. LPS. The experiment was repeated three times.