Research Paper Volume 12, Issue 10 pp 9188—9204

Figure 8. Downregulation of LINC01447 or AC106786.1 enhanced radiosensitivity in low-grade glioma cells. (A) Representative fluorescence microscope images of HS683 cells treated with FITC-siRNA-LINC01447 or FITC-siRNA-AC106786.1: blue = DAPI; green = FITC-siRNA. (B) Representative flow cytometry histograms showing cellular uptake of FITC-siRNA-LINC01447 (orange), FITC-siRNA-AC106786.1 (blue), and NC-siRNA (red). (C) Silencing efficiency was evaluated using real-time PCR. (D) CCK8 assays were used to investigate the roles of LINC01447 and AC106786.1 in HS683 cell proliferation after irradiation. (E, F) Apoptotic cells were detected by Annexin V-FITC and PI staining. Apoptosis ratio was calculated by adding early and late apoptosis percentages. (G) Colony formation efficiency was used to evaluate the radiosensitivity of treated HS683 cells. *P < 0.05; **P < 0.01; ***P < 0.001.