Research Paper Volume 12, Issue 11 pp 10246—10258

Figure 6. miR-623 inhibited XRCC5 expression and blocked cell growth by targeting XRCC5. (A) miR-623 and XRCC5 binding sites predicted by targetscan. (B) MDA-MB-453 and MCF7 cells were transfected with reporter plasmids followed by transfection with pCMV-miR-623 or pCMV-miR empty vector. The luciferase expression was analyzed as described in the “Material and Methods” section. (C, D) The XRCC5 expression was detected in NC and miR-623 cells using western blot. GAPDH was the internal control. The proliferation of (E) MDA-MB-453 cells and (F) MCF7 cells were confirmed by CCK8 assay to investigate the target of miR-623-induced cell growth block. (G, H) Transwell assay was performed to confirm the cell migration in XRCC5 and XRCC5+miR-623 cells. (I) The expression levels of protein in PI3K/AKT and Wnt/β-catenin signaling pathways were detected by western blot in (J) MDA-MB-453 cells and (K) MCF7 cells *P < 0.05 vs. XRCC5; # P < 0.05 vs. NC. P values were determined using Student’s t-tests.