Research Paper Volume 12, Issue 9 pp 8221—8240

Figure 4. R406 induces ROS generation. (A) Senescent (S) and non-senescent (NS) HDFs were treated with DMSO or R406 (10 μM) or ABT263 (1 μM) for one day and then ROS levels were detected with DHR123 staining with normalization by DNA contents. (B) Senescent and non-senescent HDFs were treated with R406 in the presence or absence of NAC for one day and then ROS levels were measured after DHR123 staining by flow cytometry. (C) Senescent HDFs were co-treated with R406 and NAC for one day and then CCK-1 assay were conducted to investigate cell viability. n = 8 (D) Senescent HDFs were co-treated with R406 and NAC for one day and then western blot assays using antibodies against caspase-9, and -7 were conducted. *p<0.05, **p<0.01, ***P<0.001