This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 12, Issue 13 pp 12684—12702
Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition
Figure 3. miR-145 suppresses the apoptosis, inflammatory reaction, and oxidative stress of VSMCs treated with Ang II. (A) the expression of the molecular marker α-SM-actin of VSMCs isolated by immunofluorescence assay (× 400); (B) miR-145 expression in VSMCs determined using RT-qPCR; (C) viability of VSMCs detected by CCK-8 assay; (D) apoptosis of VSMCs detected by flow cytometry; (E) levels of COX-2, NO, IL-1β, IL-6, and TNF-α in serum of VSMCs measured by ELISA; (F) levels of MDA and SOD in serum of VSMCs measured using kits; (G) O2- in VSMCs measured using DHE staining (× 400); (H) protein levels of cleaved caspase-3, NOX4, iNOS, p47phox, collagen I and collagen III in VSMCs determined using Western blot analysis; *, p < 0.05, vs. VSMCs without treatment; #, p < 0.05, vs. VSMCs treated with Ang II + LV-miR-NC or Ang II+LV-NC-inhibitor plasmids; measurement data were depicted as the mean ± standard deviation; comparisons among multiple groups were analyzed using one-way ANOVA followed by Turkey’s post hoc test; the experiment was repeated three times.