Figure 4.The effects of miR-375 and ATG2B on autophagy. (A) Western blot of Beclin 1, LC3 I/II and p62 after infected with miR-375 mimics, miR-375 inhibitor, pcDNA3.1-ATG2B and pcDNA3.1-ATG2B plus miR-375 mimics in OA chondrocytes; (B) Qualitative analysis of Beclin 1, LC3 I/II and p62, and the values were normalized to β-actin; (C, D) OA chondrocytes were double stained with LC3 (green) and DAPI (blue) and visualized by confocal microscopy after different treatment. * P<0.05, ** P<0.01, compared with NC group; # P<0.05, compared with pcDNA3.1-ATG2B group.
