Research Paper Volume 12, Issue 8 pp 7232—7247

Figure 1. Expression levels of a long non-coding RNA XIST and the role in neonatal mice cardiomyocytes model of anoxia. (A) The level of XIST was measured by qRT-PCR in the infarct zone of mice MI hearts compared to a sham group (n=6). The expression of XIST was significantly upregulated in MI hearts. (B) The level of XIST was measured by qRT-PCR in NMCMs treated with anoxia for 0-8 h (n=3). The expression of XIST was significantly upregulated in NMCMs under anoxia condition. (C) The level of XIST RNA in NMCMs after transfected with XIST siRNAs (S1-S3) and siRNA control. All XIST siRNAs successfully knocked down the expression of XIST (n=3). (D) Cell viability was detected using the MTT assay (n=3). (E) Apoptotic cells were analyzed by flow cytometry after anoxia treatment (n=3). Anoxia condition was induced by placing cells in hypoxia chamber for 8 h. Control: normal culture, Sc: the siRNA control. Data are shown as mean ± SD, * P<0.05.