Research Paper Volume 12, Issue 8 pp 6947—6965
Diabetes-induced glucolipotoxicity impairs wound healing ability of adipose-derived stem cells-through the miR-1248/CITED2/HIF-1α pathway
Figure 1. Characterization of hADSCs and the proliferation ability of the three hADSCs against glucolipotoxicity. (A) Flow cytometric analysis of extracted hADSCs. Cells were positive for CD29 and CD90 markers, and negative for CD31, CD34 and CD45 markers; (B) The proliferation of three different hADSCs by CCK-8 assay; (C) Wound healing assays to detect the migration ability of hADSCs; (D) Transwell assays to detect the invasion ability of hADSCs; (E) The mRNA expression of the migration-related, including CXCR4, MMP2 and MMP9, was detected by RT-qPCR analysis; (F) The protein expression of the migration-related, including CXCR4, MMP2 and MMP9, was detected by western blot analysis (* P<0.05).