Figure 1. (A) let-7f-5p expression was decreased in osteosarcoma (OS) cell lines. qRT-PCR was used to evaluate the endogenous levels of let-7f-5p in hFOB cells and HOS, U2OS, and Saos OS cell lines. (B) and (C) qRT-PCR was used to quantitate the expression of let-7f-5p following transfection with the let-7f-5p mimic or inhibitor in Saos cells.