Research Paper Volume 12, Issue 8 pp 6865—6879

Figure 5. miR-548 and KIF2C are downstream targets of circRGNEF. (A) Bioinformatics analysis (https://circinteractome.nia.nih.gov/bin/mirnasearch) and high-throughput sequencing indicated miR-548 is a target of circRGNEF. (B) RT-qPCR assay of miR-548 in 90 paired BC tumor and adjacent non-tumor tissues. Data are means ± SD. ***P < 0.001 vs. normal controls. (C) The mutated (Mut) version of circRGNEF is shown. (D) The relative luciferase activity was determined 48 h after transfection of HEK293T cells with miR-548 mimic/normal control (NC) or circRGNEF wild-type/Mut. Data are presented as the mean ± SD. ^***P < 0.001. (E) Bioinformatics analysis (http://circnet.mbc.nctu.edu.tw/ and http://www.targetscan.org/vert_71/) indicated KIF2C is a direct target of miR-548. (F) The mutated (Mut) version of the 3’UTR-KIF2C is shown. (G) The relative luciferase activity was determined 48 h after transfection of HEK293T cells with miR-548 mimic/normal control (NC) or 3’UTR-KIF2C wild-type/Mut. Data are presented as the mean ± SD. ^***P < 0.001.