Research Paper Volume 12, Issue 8 pp 6793—6807

Figure 6. microRNA-708 (miR-708) suppression attenuates the effects of long intergenic nonprotein-coding RNA 00514 (LINC00514) silencing in OS cells. Si-LINC00514 together with in-miR-708 or in-miR-NC was introduced into HOS and MG-63 cells. The transfected cells were subjected to Cell Counting Kit-8 assay, colony formation assay, flow cytometry, and transwell migration and invasion assays to assess cell proliferation (A), colony formation (B), apoptosis (C), cell cycle (D), migration (E), and invasion (F) in vitro. *P < 0.05 and **P < 0.01.