Figure 6. Impact of RMF on Treg cells. (A) Proportions of Treg cells in the spleen as well as lymph nodes were determined. (B) Heat map of TGF-β, Foxp3 and IL-10 expression difference in the spinal cord. (C) The manifestation of TGF-β, Foxp3 and IL-10 mRNA in spinal cord was measured by qPCR. Data are shown as the mean ± SEM of three independent experiments. *P <0.05, **P <0.01.