Research Paper Volume 12, Issue 8 pp 6644—6666

Figure 2. Dox treatment induces autophagy and Sox2OT-V7 expression in U2OS cells. (A) U2OS cells were treated with 1, 2.5, or 5 μM Dox or 2 μM rapamycin, and the expression of LC3II, Beclin 1, and p62 was measured. U2OS cells with stable eGFP-LC3 expression were treated with Dox (5 μM) or rapamycin (2 μM) for 24 h. Autophagy was examined by transmission electron microscopy (B) puncta were imaged by using a confocal microscope, and representative images are presented (C). (D) U2OS cells were treated with 1, 2.5 or 5 μM Dox and examined for the expression of Sox2OT-V7. (E) U2OS cells were treated with 0.001, 0.01, 0.1, 1, 10, and 100 μM Dox in the presence or absence of the autophagy inhibitor 3-MA and examined for cell viability (IC₅₀ value). The data are presented as the mean ± SD of three independent experiments. **P<0.01.