Research Paper Volume 12, Issue 8 pp 6611—6629

Figure 6. HO-1 inhibition enhances bortezomib-induced antiproliferative effect by reducing Gas6 secretion. (A) Gas6 mRNA level in human primary CD138⁺ cells. (B) Gas6 levels of culture supernatants were quantified by ELISA. Human primary CD138⁺ cells were treated with or without ZnPPIX (0.1 μM) in the presence of bortezomib for 24 h. n=5, ^*P<0.05 vs. control group (ZnPPIX = 0 μM). (C) CCK8 showing the cell viability of CD138⁺ cells treated with ZnPPIX, exogenous Gas6 or Gas6 neutralizing antibody. n=5, ^*P<0.05. (D–G) Cell viability was detected by CCK8 assays in U266 and RPMI8226 cells. n=4, ^*P<0.05.