Research Paper Volume 12, Issue 7 pp 5992—6017

Figure 7. ELK1 transcriptionally induces KIAA0101 expression. (A) Venn diagram of transcription factors prediction of KIAA0101 and TRIP4 by PROMO and QIAGEN. (B) Representative sequence logo of ELK-1 binding specificity queried from the Jaspar²⁰¹⁸, (identifier MA0028.1). (C) ELK1 expression and its association with tumour grade in HNSC were analysed by UALCAN. (D, E) ChIP assay was performed in CNE-2Z cells using anti-ELK1, and normal IgG. Input of sheared chromatin was prepared prior to immunoprecipitation. **p<0.01, ****p<0.0001. (F) Relative expression of KIAA0101 in CNE-2Z cells after siELK1 treatment. Histogram is the average ratio (mean ± SD) of three independent experiments. **p<0.001, *p<0.005 by Student’s t test. (G) MAPK-ELK1 signalling pathway revealed by cBioPortal. Network view of the ELK1 neighbourhood in HNSC. The network contains 19 nodes, including 1 query gene (green node) and the 18 most frequently altered neighbour genes. The depth of colour represents the degree of alteration.