Research Paper Volume 12, Issue 7 pp 5948—5976

Figure 13. miR-10a-5p functioned in IL-1β induced Hc-a through targeting HOXA3. Hc-a was treated with IL-1β and then co-transfected miR-10a-5p inhibitor and si-HOXA3. (A) HOXA3, Col2a1, MMP13, BAX, and GAPDH protein levels were detected by western blot. (B, D, F) cell proliferation detected by CCK8 (B; n=3; *P < 0.05, ***P < 0.001) and EDU assay (D, F; n=3, *P < 0.05, **P < 0.01, ***P < 0.001). (C, E) cell apoptosis detected by flow cytometry assay (n=3, **P < 0.01, ***P < 0.001).