Figure 5.Compound 13i HCl increases MLKL phosphorylation and ROS production in bladder cancer cells. (A, B) RT112 cells were treated with the indicated concentrations of 13i HCl (A) or PF-670462 (B) for 24 h, 48 h and 72 h. The cells were subjected to Western blotting with the indicated antibodies. (C) RT112 cells were exposed to 13i HCl for 72 h and LDH levels in the supernatants were determined. (D, E) RT112 cells were exposed to the indicated concentrations of 13i HCl for 72 h or H2O2 (10 μM) for 1 h. The cells were stained with H2DCFDA (D) or MitoSOX (E) and subjected to flow cytometry. Data are represented as mean ± S.D. *P<0.05, **P<0.01, ***P<0.001 compared to control cells (n=3).
