Research Paper Volume 12, Issue 7 pp 5640—5650
Inhibition of miR-19a partially reversed the resistance of colorectal cancer to oxaliplatin via PTEN/PI3K/AKT pathway
Figure 6. Anti-miR-19a enhanced the oxaliplatin-induced apoptosis through the PTEN/PI3K/AKT pathway. (A) Western blot assays were performed to evaluate the effect of anti-miR-19a (50 pmol/ml) and siRNA-PTEN (50 pmol/ml) on affecting the phosphorylation of PI3K and AKT in SW480/R and HT29/R cells. (B) Western blot assays were performed to evaluate the effect of anti-miR-19a (50 pmol/ml) and siRNA-PTEN (50 pmol/ml) on affecting the activation of caspase-9, -7 and -3 which was dependent by oxaliplatin in SW480/R and HT29/R cells. (C) Anti-miR-19a (50 pmol/ml) increased the apoptotic rate of SW480/R and HT29/R cells which were treated with oxaliplatin (10 μM). *P<0.05 vs. oxaliplatin+miR-c group. #P<0.05 vs. oxaliplatin+anti-miR-19a group.