Research Paper Volume 12, Issue 4 pp 3662—3681

Figure 6. Cell fate following MF exposure. G401and A549 cells were subjected to MF or sham exposure, 2 h daily for 3 consecutive days. (A, B) EdU incorporation assay to detect the ratio of EdU positive nuclei (A), and ratio of nuclei with partial EdU incorporation (B). (C) Cell apoptosis rates measured by flow cytometry. (D) Expression of PARP and caspase 3 (precursor and cleaved forms) in G401 nephroblastoma xenografts established in nude mice. (E) Ferroptosis detected by co-incubation with ferrostatin-1 (Fer-1, 0.5 μM, 12 h per day) together with MF exposure in G401 and A549. Results are expressed as mean ± SE from 3 independent experiments (n=5 in each experiment). *P < 0.05, **: P<0.01; ***: P<0.001.