Research Paper Volume 12, Issue 4 pp 3574—3593

Figure 7. SERPINH1 expression regulates in vitro migration and invasion of GC cells. (A) Wound healing assay shows that the distance between wound edges was higher in SERPINH1-silenced SGC-7901 cells than in the control SGC-7901 cells at 24 h. Conversely, the distance between wound edges was significantly lower in the SERPINH1-overexpressing MGC-803 cells than in the control MGC-803 cells at 24 h. (B) Quantitative analysis of wound healing assay in the control and SERPINH1-silenced SGC-7901 cells, as well as control and SERPINH1-overexpressing MGC-803 cells. (C) Representative images show results of the Transwell migration assay, and (D) histogram plots show the number of migrating cells in the control and SERPINH1-silenced SGC-7901 cells, as well as control and SERPINH1-overexpressing MGC-803 cells. As shown, migration is reduced in SERPINH1-silenced SGC-7901 cells and increased in SERPINH1 overexpressed MGC-803 cells compared with the corresponding controls. (E) Western blot analysis shows that MMP2 and MMP9 protein levels are significantly reduced in the shSERPINH1-silenced SGC-7901 cells and increased in the SERPINH1-overexpressing MGC-803 cells compared with the corresponding controls. (F, G) Gene expression analysis shows that (F) MMP2 (r=0.55, P<0.0001) and (G) MMP9 (r=0.24, P<0.0001) mRNA levels positively correlate with SERPINH1 mRNA levels in GC patients from the TCGA-STAD dataset.