Research Paper Volume 12, Issue 4 pp 3218—3237

Figure 4. miR-101 is involved in LINC00623 regulating HRAS. (A) Previously reported miRNAs related to chondrocyte apoptosis were analyzed; the online tool LncTar was used to identify miRNAs that could be targeted by LINC00623; and miR-101-5p was selected. (B) The expression of miR-101 in normal, mild OA and severe OA tissues was examined using real-time PCR. (C) OA chondrocytes were treated with PBS or IL-1β and then examined for miR-101 expression using real-time PCR. (D) miR-101 expression was regulated by transfection of miR-101 mimics or miR-101 inhibitor, and was confirmed using real-time PCR. (E) LINC00623 mRNA expression in response to miR-101 overexpression and miR-101 inhibition was examined using real-time PCR. (F) miR-101 expression in response to LINC00623 knockdown was examined using real-time PCR. (G) HRAS protein levels in response to miR-101 overexpression and miR-101 inhibition were examined using immunoblotting. The data are presented as mean ± SD of three independent experiments. **P<0.01.